Journal: Cancers

Article Title: Renal Function Parameters in Distinctive Molecular Subtypes of Prostate Cancer

doi: 10.3390/cancers15205013

Figure Lengend Snippet: Characteristics of primary antibodies.

Article Snippet: anti-SPINK1 , mouse monoclonal antibody, Abnova, clone 4D4, H00006690-M01, Taipei, Taiwan , 1:100 , cytoplasmic.

Techniques: Expressing

Journal: Microbial Biotechnology

Article Title: Immunoassay Detection of SARS ‐ CoV ‐2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

doi: 10.1111/1751-7915.70117

Figure Lengend Snippet: Direct ELISA data reporting the binding of 4D4 (panel A) and 1H6 (panel B) MAbs to hCoV NP. MAbs were conjugated to HRP and binding to 1 μg/mL of recombinant viral nucleocapsid protein (NP) reported with an enhanced chemiluminescent substrate (ECL) and a luminometer. Binding data is expressed as the mean ± SEM in counts per second (CPS) with BSA and no antigen (dashed red line) controls. See recombinant NP details in Table [2–9]. Key: [2] SARS‐CoV‐2 B.1.1.529 from HEK 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.

Article Snippet: A BioDot XYZ3060 instrument (BioDot, CA) fitted with a non‐contact BioJet valve was used to dispense 40 nL drops at 1 μL/cm for the test line (T), 4D4 MAb at 3 mg/mL, and the control line (C), donkey‐anti‐mouse‐IgG (H + L) at 1 mg/mL (Jackson ImmunoResearch, MA) on Hi‐Flow Plus 90 nitrocellulose membrane cards (Millipore, MA).

Techniques: Direct ELISA, Binding Assay, Recombinant

Journal: Microbial Biotechnology

Article Title: Immunoassay Detection of SARS ‐ CoV ‐2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

doi: 10.1111/1751-7915.70117

Figure Lengend Snippet: Western blots showing the detection of disulfide reduced and heat denatured recombinant viral nucleocapsid proteins (NP) after electrophoresis (1 μg) with 4D4 (panel A) and 1H6 (panel B) MAb. Images were acquired at 30 s from nitrocellulose blots using a chemiluminescent substrate with HRP‐conjugated MAb (1 μg/mL) detection. A mixed standard was used to define protein migration by molecular weight (kDa). See recombinant NP details in Table [1–9]. Key: [1] SARS‐CoV‐2B 1.1.529 from E. coli . [2] SARS‐CoV‐2 B.1.1.529 from HEK 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.

Article Snippet: A BioDot XYZ3060 instrument (BioDot, CA) fitted with a non‐contact BioJet valve was used to dispense 40 nL drops at 1 μL/cm for the test line (T), 4D4 MAb at 3 mg/mL, and the control line (C), donkey‐anti‐mouse‐IgG (H + L) at 1 mg/mL (Jackson ImmunoResearch, MA) on Hi‐Flow Plus 90 nitrocellulose membrane cards (Millipore, MA).

Techniques: Western Blot, Recombinant, Electrophoresis, Migration, Molecular Weight

Journal: Microbial Biotechnology

Article Title: Immunoassay Detection of SARS ‐ CoV ‐2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

doi: 10.1111/1751-7915.70117

Figure Lengend Snippet: Sandwich ELISA data plotting dose–response of 4D4 and 1H6‐HRP binding to the SARS‐CoV‐2 Omicron B.1.1.529 NP variant. Chemiluminescent detection (panel A) is reported in counts per second (CPS) and colorimetric TMB detection in absorbance at 650 nm (panel B). Data points (open circles) represent the mean ± SEM. The solid line represents 4PL curve fit data and the vertical dashed line plots the assay EC50 value. Assay LODs are 15.3 pg/mL ( p = < 0.025).

Article Snippet: A BioDot XYZ3060 instrument (BioDot, CA) fitted with a non‐contact BioJet valve was used to dispense 40 nL drops at 1 μL/cm for the test line (T), 4D4 MAb at 3 mg/mL, and the control line (C), donkey‐anti‐mouse‐IgG (H + L) at 1 mg/mL (Jackson ImmunoResearch, MA) on Hi‐Flow Plus 90 nitrocellulose membrane cards (Millipore, MA).

Techniques: Sandwich ELISA, Binding Assay, Variant Assay